Preprocessing of the Metabolome and Microbiome data

Preprocessing
Use Phonetype in the preprocessing?
Categorical phenotypes will be preprocessed by groups.
Methods of Preprocessing
Metabolome Microbiome
Using "KNN" imputation method in "DMwR" R package in default. "QRILC" in "imputeLCMD" R package, a missing data imputation method that performs the imputation of left-censored missing data using random draws from a truncated distribution with parameters estimated using quantile regression.
Using "KNN" imputation method in "DMwR" R package in default. "QRILC" in "imputeLCMD" R package, a missing data imputation method that performs the imputation of left-censored missing data using random draws from a truncated distribution with parameters estimated using quantile regression.
Methods of normalization, the sum of each sample is 30000.
Methods of normalization, the sum of each sample is 30000.
The logarithmic transformation of base e.
Method of CLR transformation.
Rarefaction method in "phyloseq" R package.